Hotspot

rbcL-accD

LSC · 56098-56904 · 807 bp

Back to Renanthera

Pi 0.0392

MarkerSeek score 75.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 62
pi: 0.0392
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTTCGACCCAGTGGATAAGC	AGACAACATTGAATTGAACCA	50.0	33.3	57.6	53.9	872-882 bp	1.0	46.9
rbcL-accD_p2	TTTCGACCCAGTGGATAAGC	AGACAACATTGAATTGAACCAA	50.0	31.8	57.6	54.7	872-882 bp	1.0	46.9
rbcL-accD_p3	TTTCGACCCAGTGGATAAGC	AGACAACATTGAATTGAACCAAT	50.0	30.4	57.6	55.3	872-882 bp	1.0	46.9
rbcL-accD_p4	TTTCGACCCAGTGGATAAGC	GACAACATTGAATTGAACCA	50.0	35.0	57.6	52.2	871-881 bp	1.0	46.9
rbcL-accD_p5	ATTTCGACCCAGTGGATAAGC	AGACAACATTGAATTGAACCA	47.6	33.3	58.1	53.9	873-883 bp	1.0	46.9