Hotspot

trnF-GAA-trnV-UAC

LSC · 50121-50697 · 577 bp

Back to Renanthera

Pi 0.0326

MarkerSeek score 76.10

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 34
pi: 0.0326
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	633-2382 bp	1.0	83.1
trnF-GAA-trnV-UAC_p2	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	632-2381 bp	1.0	80.4
trnF-GAA-trnV-UAC_p3	AATCCTCGTGTCACCAGTTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	636-2385 bp	1.0	79.9
trnF-GAA-trnV-UAC_p4	TCGGGATAGCTCAGTTGGTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	671-2420 bp	1.0	79.8
trnF-GAA-trnV-UAC_p5	CCTCGTGTCACCAGTTCAAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.1	58.7	621-2370 bp	1.0	79.3