Hotspot

psbM-trnD-GUC

LSC · 31208-32346 · 1139 bp

Back to Renanthera

Pi 0.0196

MarkerSeek score 67.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 40
pi: 0.0196
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1011-1263 bp	1.0	85.4
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1016-1268 bp	1.0	83.9
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1068-1320 bp	1.0	83.8
psbM-trnD-GUC_p4	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1015-1267 bp	1.0	83.0
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1017-1269 bp	1.0	82.2