Hotspot

rpoB-trnC-GCA

LSC · 27510-29318 · 1809 bp

Back to Renanthera

Pi 0.0412
MarkerSeek score 74.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1809
variable_sites
104
pi
0.0412
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.88
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTGGACATTCCCTCATTTCCA CCGGGGATAAAGGATTTGCA 47.6 50.0 57.9 57.9 1377-1892 bp 1.0 79.8
rpoB-trnC-GCA_p2 TGTGGACATTCCCTCATTTCC CCGGGGATAAAGGATTTGCA 47.6 50.0 57.9 57.9 1378-1893 bp 1.0 79.8
rpoB-trnC-GCA_p3 GTGGACATTCCCTCATTTCCA ACCGGGGATAAAGGATTTGC 47.6 50.0 57.9 57.9 1378-1893 bp 1.0 79.8
rpoB-trnC-GCA_p4 TGTGGACATTCCCTCATTTCC ACCGGGGATAAAGGATTTGC 47.6 50.0 57.9 57.9 1379-1894 bp 1.0 79.8
rpoB-trnC-GCA_p5 GTGGACATTCCCTCATTTCCA TCGAACCGGGGATAAAGGAT 47.6 50.0 57.9 58.2 1382-1897 bp 1.0 78.7