Hotspot

trnR-UCU-atpA

LSC · 10807-10962 · 156 bp

Back to Renanthera

Pi 0.0556

MarkerSeek score 76.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 16
pi: 0.0556
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CGGCAGCTCAAATTAAAGCC	50.0	50.0	58.0	58.1	826-859 bp	1.0	84.7
trnR-UCU-atpA_p2	AGCCTTCCAAGCTAACGATG	CCCTGGTCGTGAAGCTTATC	50.0	55.0	58.0	58.1	1089-1122 bp	1.0	83.7
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	CCTGGTCGTGAAGCTTATCC	50.0	55.0	58.0	58.1	1088-1121 bp	1.0	83.7
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1085-1118 bp	1.0	83.7
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	GGTCGTGAAGCTTATCCAGG	55.0	55.0	58.1	58.1	1088-1121 bp	1.0	83.5