Hotspot

psbI-trnS-GCU

LSC · 8238-8411 · 174 bp

Back to Renanthera

Pi 0.0326

MarkerSeek score 67.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 10
pi: 0.0326
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.6	58.2	211-220 bp	1.0	82.8
psbI-trnS-GCU_p2	CTTATCTAACGATCCGGGGC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	57.6	58.2	231-240 bp	1.0	82.7
psbI-trnS-GCU_p3	CTTATCTAACGATCCGGGGC	TTCGAATCCCTCTCTCTCCG	55.0	55.0	57.6	58.3	228-237 bp	1.0	82.0
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	57.6	58.3	208-217 bp	1.0	82.0
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAAT	GGGTTCGAATCCCTCTCTCT	47.6	55.0	58.1	58.2	211-220 bp	1.0	77.3