Hotspot

psbA-trnK-UUU

LSC · 1159-1396 · 238 bp

Back to Renanthera

Pi 0.0406

MarkerSeek score 71.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 17
pi: 0.0406
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.0	58.6	315-325 bp	1.0	80.5
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.0	58.8	305-315 bp	1.0	78.9
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.0	58.8	306-316 bp	1.0	78.9
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.0	58.9	310-320 bp	1.0	78.4
psbA-trnK-UUU_p5	GCTTGTACTTTCGCGTCTCT	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.3	58.6	312-322 bp	1.0	78.3