Hotspot

ycf1

SSC · 108202-113238 · 5037 bp

Back to Reichardia

Pi 0.00837

MarkerSeek score 44.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5037
variable_sites: 104
pi: 0.00837
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTGAGACTCAAAACAAAAAGA	GGATAGGGAAGAAGGATATCATGA	34.8	41.7	57.1	56.9	183-195 bp	1.0	47.8
ycf1_p2	TGCTGAGACTCAAAACAAAAAGAA	GGATAGGGAAGAAGGATATCATGA	33.3	41.7	57.7	56.9	183-195 bp	1.0	47.8
ycf1_p3	TGCTGAGACTCAAAACAAAAAGA	GGATAGGGAAGAAGGATATCATGAA	34.8	40.0	57.1	57.5	183-195 bp	1.0	47.8
ycf1_p4	TGCTGAGACTCAAAACAAAAAGA	GGATAGGGAAGAAGGATATCATG	34.8	43.5	57.1	55.5	183-195 bp	1.0	47.8
ycf1_p5	TGCTGAGACTCAAAACAAAAAG	GGATAGGGAAGAAGGATATCATGA	36.4	41.7	55.7	56.9	183-195 bp	1.0	47.8