Hotspot

psbA

LSC · 470-1531 · 1062 bp

Back to Reichardia

Pi 0.00104

MarkerSeek score 29.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AGATATTGGTTGACACGGGC	55.0	50.0	58.1	58.0	1591-1596 bp	1.0	80.5
psbA_p2	CAATCCACTGCCTTGATCCA	AGATATTGGTTGACACGGGC	50.0	50.0	57.9	58.0	1605-1610 bp	1.0	80.4
psbA_p3	AATCCACTGCCTTGATCCAC	AGATATTGGTTGACACGGGC	50.0	50.0	57.9	58.0	1604-1609 bp	1.0	80.4
psbA_p4	ACAATCCACTGCCTTGATCC	AGATATTGGTTGACACGGGC	50.0	50.0	57.9	58.0	1606-1611 bp	1.0	80.4
psbA_p5	GATCCACTTGGCTACATCCG	GAAAGCCGTGTGCAATGAAA	55.0	45.0	58.1	57.9	1819-1822 bp	1.0	79.7