Hotspot

psaB

LSC · 36868-39072 · 2205 bp

Back to Reichardia

Pi 0.00175

MarkerSeek score 51.70

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 11
pi: 0.00175
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	TCCATGATACAAGTCGGATCC	ACAACATGGGCGTTCTTCTT	47.6	45.0	57.0	58.0	2381-2386 bp	1.0	64.2
psaB_p2	CCATGATACAAGTCGGATCCT	ACAACATGGGCGTTCTTCTT	47.6	45.0	57.0	58.0	2380-2385 bp	1.0	64.1
psaB_p3	TCCATGATACAAGTCGGATCC	TACAAGGACGTGCTGTAGGA	47.6	50.0	57.0	58.1	2431-2436 bp	1.0	63.6
psaB_p4	GGTGGATCTCTTCTTCATCGA	ACAACATGGGCGTTCTTCTT	47.6	45.0	56.9	58.0	2340 bp	1.0	63.5
psaB_p5	AGGTGGATCTCTTCTTCATCG	ACAACATGGGCGTTCTTCTT	47.6	45.0	56.9	58.0	2341 bp	1.0	63.5