Hotspot

trnN-trnR

IRa · 127961-128465 · 505 bp

Back to Reichardia

Pi 0.00438

MarkerSeek score 54.20

Primer available yes

Label trnN-trnR

Feature Metadata

label_name: trnN-trnR
feature_type: igs
region: IRa
length_bp: 505
variable_sites: 5
pi: 0.00438
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-trnR_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	608-648 bp	1.0	82.9
trnN-trnR_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	645-685 bp	1.0	82.3
trnN-trnR_p3	GGTTGAGAACGGGAATGGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	647-687 bp	1.0	81.3
trnN-trnR_p4	GGTTGAGAACGGGAATGGAA	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	684-724 bp	1.0	80.7
trnN-trnR_p5	TCTCCCGTTGTTCCTCAGTA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	612-652 bp	1.0	80.5