Hotspot

rpl32-ndhF

SSC · 123754-124840 · 1087 bp

Back to Reichardia

Pi 0.00979

MarkerSeek score 50.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1087
variable_sites: 24
pi: 0.00979
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1262-1294 bp	1.0	79.0
rpl32-ndhF_p2	CCGCCCAATATCCTTTCCTT	TAGGAACTGGGAGTGGAAGG	50.0	55.0	57.6	58.0	1222-1254 bp	1.0	79.0
rpl32-ndhF_p3	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1260-1292 bp	1.0	78.9
rpl32-ndhF_p4	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1261-1293 bp	1.0	78.9
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1279-1311 bp	1.0	78.8