Hotspot

N4L36_pgt030-rpl32

SSC · 122848-123588 · 741 bp

Back to Reichardia

Pi 0.0133
MarkerSeek score 59.50
Primer available yes
Label N4L36_pgt030-rpl32

Feature Metadata

label_name
N4L36_pgt030-rpl32
feature_type
igs
region
SSC
length_bp
741
variable_sites
22
pi
0.0133
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
N4L36_pgt030-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 873-1041 bp 1.0 80.3
N4L36_pgt030-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 874-1042 bp 1.0 80.2
N4L36_pgt030-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 856-1024 bp 1.0 80.1
N4L36_pgt030-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 857-1025 bp 1.0 80.1
N4L36_pgt030-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAGGAAAGGATATTGGGCGG 50.0 50.0 57.9 57.6 883-1051 bp 1.0 79.3