Hotspot

trnR-trnN

IRb · 107297-107801 · 505 bp

Back to Reichardia

Pi 0.00438

MarkerSeek score 54.20

Primer available yes

Label trnR-trnN

Feature Metadata

label_name: trnR-trnN
feature_type: igs
region: IRb
length_bp: 505
variable_sites: 5
pi: 0.00438
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnN_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	608-648 bp	1.0	82.9
trnR-trnN_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	645-685 bp	1.0	82.3
trnR-trnN_p3	CAGAGGATTAGAGCACGTGG	GGTTGAGAACGGGAATGGAA	55.0	50.0	57.8	57.8	647-687 bp	1.0	81.3
trnR-trnN_p4	GAACAGATCCAGTGGAGACG	GGTTGAGAACGGGAATGGAA	55.0	50.0	57.7	57.8	684-724 bp	1.0	80.7
trnR-trnN_p5	CAGAGGATTAGAGCACGTGG	TCTCCCGTTGTTCCTCAGTA	55.0	50.0	57.8	57.7	612-652 bp	1.0	80.5