Hotspot

ycf4-cemA

LSC · 60369-61007 · 639 bp

Back to Reichardia

Pi 0.00598
MarkerSeek score 49.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
639
variable_sites
9
pi
0.00598
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 GAGCCATTCCATTGACTCGT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 819-983 bp 1.0 80.9
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.1 58.1 751-915 bp 1.0 80.5
ycf4-cemA_p3 CTCCACGGGAAATGGAACAA GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 783-947 bp 1.0 80.4
ycf4-cemA_p4 AAAAAGCTGCTGAATTGGCC GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 765-929 bp 1.0 79.8
ycf4-cemA_p5 AAAGCTGCTGAATTGGCCTA GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 763-927 bp 1.0 79.5