Hotspot

N4L36_pgt008-rpoB

LSC · 11979-12907 · 929 bp

Back to Reichardia

Pi 0.0124

MarkerSeek score 64.40

Primer available yes

Label N4L36_pgt008-rpoB

Feature Metadata

label_name: N4L36_pgt008-rpoB
feature_type: igs
region: LSC
length_bp: 929
variable_sites: 25
pi: 0.0124
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
N4L36_pgt008-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	1049-1077 bp	1.0	67.0
N4L36_pgt008-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	1008-1036 bp	1.0	66.5
N4L36_pgt008-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1039-1067 bp	1.0	66.2
N4L36_pgt008-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1044-1072 bp	1.0	66.2
N4L36_pgt008-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	1039-1067 bp	1.0	65.7