Hotspot

psbM-trnD

LSC · 10801-11461 · 661 bp

Back to Reichardia

Pi 0.00501

MarkerSeek score 51.30

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 9
pi: 0.00501
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	762-780 bp	1.0	53.2
psbM-trnD_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	756-774 bp	1.0	52.8
psbM-trnD_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.4	58.3	759-777 bp	1.0	51.8
psbM-trnD_p4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.3	58.3	763-781 bp	1.0	51.2
psbM-trnD_p5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	36.4	50.0	56.2	58.3	763-781 bp	1.0	50.3