Hotspot

trnH-psbA

LSC · 78-469 · 392 bp

Back to Reichardia

Pi 0.0316

MarkerSeek score 60.90

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 26
pi: 0.0316
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	505-510 bp	1.0	81.3
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	519-524 bp	1.0	81.2
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	518-523 bp	1.0	81.2
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	520-525 bp	1.0	81.1
trnH-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	506-511 bp	1.0	80.8