primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	469	505-510	1.000000	81.3
trnH-psbA_p2	trnH-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	469	519-524	1.000000	81.2
trnH-psbA_p3	trnH-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	469	518-523	1.000000	81.2
trnH-psbA_p4	trnH-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	469	520-525	1.000000	81.1
trnH-psbA_p5	trnH-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	469	506-511	1.000000	80.8
psbA_p1	psbA	1	GATCCACTTGGCTACATCCG	AGATATTGGTTGACACGGGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.167307	470	1531	1591-1596	1.000000	80.5
psbA_p2	psbA	2	CAATCCACTGCCTTGATCCA	AGATATTGGTTGACACGGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.171668	470	1531	1605-1610	1.000000	80.4
psbA_p3	psbA	3	AATCCACTGCCTTGATCCAC	AGATATTGGTTGACACGGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.173176	470	1531	1604-1609	1.000000	80.4
psbA_p4	psbA	4	ACAATCCACTGCCTTGATCC	AGATATTGGTTGACACGGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.173176	470	1531	1606-1611	1.000000	80.4
psbA_p5	psbA	5	GATCCACTTGGCTACATCCG	GAAAGCCGTGTGCAATGAAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.261660	470	1531	1819-1822	1.000000	79.7
N4L36_pgt004-trnC_p1	N4L36_pgt004-trnC	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8615	9349	884-887	1.000000	79.6
N4L36_pgt004-trnC_p2	N4L36_pgt004-trnC	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8615	9349	866-869	1.000000	77.4
N4L36_pgt004-trnC_p3	N4L36_pgt004-trnC	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8615	9349	820-823	1.000000	75.8
N4L36_pgt004-trnC_p4	N4L36_pgt004-trnC	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8615	9349	885-888	1.000000	75.4
N4L36_pgt004-trnC_p5	N4L36_pgt004-trnC	5	ACGGAAAGAGAGGGATTCGA	AAATCCCCTGTTGATGAGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.301321	8615	9349	911-911	0.125000	42.6
trnC-petN_p1	trnC-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9422	10132	768-877	1.000000	80.8
trnC-petN_p2	trnC-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9422	10132	786-895	1.000000	78.6
trnC-petN_p3	trnC-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9422	10132	767-876	1.000000	74.9
trnC-petN_p4	trnC-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9422	10132	776-885	1.000000	74.8
trnC-petN_p5	trnC-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9422	10132	825-934	1.000000	74.0
psbM-trnD_p1	psbM-trnD	1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10801	11461	762-780	1.000000	53.2
psbM-trnD_p2	psbM-trnD	2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	10801	11461	756-774	1.000000	52.8
psbM-trnD_p3	psbM-trnD	3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.4	58.3	0.0	0.0	3.875520	10801	11461	759-777	1.000000	51.8
psbM-trnD_p4	psbM-trnD	4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.3	58.3	0.0	0.0	3.958013	10801	11461	763-781	1.000000	51.2
psbM-trnD_p5	psbM-trnD	5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	22	20	36.4	50.0	56.2	58.3	0.0	0.0	4.069095	10801	11461	763-781	1.000000	50.3
N4L36_pgt008-rpoB_p1	N4L36_pgt008-rpoB	1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.0	56.8	0.0	0.0	2.237066	11979	12907	1049-1077	1.000000	67.0
N4L36_pgt008-rpoB_p2	N4L36_pgt008-rpoB	2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.1	56.8	14.8	0.0	2.326325	11979	12907	1008-1036	1.000000	66.5
N4L36_pgt008-rpoB_p3	N4L36_pgt008-rpoB	3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	11979	12907	1039-1067	1.000000	66.2
N4L36_pgt008-rpoB_p4	N4L36_pgt008-rpoB	4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	11979	12907	1044-1072	1.000000	66.2
N4L36_pgt008-rpoB_p5	N4L36_pgt008-rpoB	5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.0	57.6	0.0	0.0	2.409970	11979	12907	1039-1067	1.000000	65.7
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	25108	26238	1267-1270	1.000000	78.8
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	25108	26238	1323-1326	1.000000	78.2
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	25108	26238	1283-1286	1.000000	77.9
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	25108	26238	1268-1271	1.000000	77.7
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	25108	26238	1327-1330	1.000000	77.5
psaB_p1	psaB	1	TCCATGATACAAGTCGGATCC	ACAACATGGGCGTTCTTCTT	21	20	47.6	45.0	57.0	58.0	0.0	0.0	2.048350	36868	39072	2381-2386	1.000000	64.2
psaB_p2	psaB	2	CCATGATACAAGTCGGATCCT	ACAACATGGGCGTTCTTCTT	21	20	47.6	45.0	57.0	58.0	10.8	0.0	2.056325	36868	39072	2380-2385	1.000000	64.1
psaB_p3	psaB	3	TCCATGATACAAGTCGGATCC	TACAAGGACGTGCTGTAGGA	21	20	47.6	50.0	57.0	58.1	0.0	11.4	2.115829	36868	39072	2431-2436	1.000000	63.6
psaB_p4	psaB	4	GGTGGATCTCTTCTTCATCGA	ACAACATGGGCGTTCTTCTT	21	20	47.6	45.0	56.9	58.0	0.0	0.0	2.104310	36868	39072	2340-2340	1.000000	63.5
psaB_p5	psaB	5	AGGTGGATCTCTTCTTCATCG	ACAACATGGGCGTTCTTCTT	21	20	47.6	45.0	56.9	58.0	0.0	0.0	2.112392	36868	39072	2341-2341	1.000000	63.5
ycf3-N4L36_pgt015_p1	ycf3-N4L36_pgt015	1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	44017	44939	1031-1033	1.000000	71.4
ycf3-N4L36_pgt015_p2	ycf3-N4L36_pgt015	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	44017	44939	981-983	1.000000	71.1
ycf3-N4L36_pgt015_p3	ycf3-N4L36_pgt015	3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	21	20	47.6	55.0	58.0	57.8	8.2	0.0	1.260963	44017	44939	1019-1021	1.000000	70.9
ycf3-N4L36_pgt015_p4	ycf3-N4L36_pgt015	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	44017	44939	983-985	1.000000	70.6
ycf3-N4L36_pgt015_p5	ycf3-N4L36_pgt015	5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	21	20	47.6	50.0	58.0	57.6	8.2	0.0	1.437074	44017	44939	1009-1011	1.000000	69.5
ndhC-trnV_p1	ndhC-trnV	1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	4.6	0.0	0.246856	50210	51388	1321-1341	1.000000	80.3
ndhC-trnV_p2	ndhC-trnV	2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.7	58.1	4.6	0.0	0.418656	50210	51388	1312-1332	1.000000	79.0
ndhC-trnV_p3	ndhC-trnV	3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.4	58.1	0.0	0.0	0.466616	50210	51388	1329-1349	1.000000	78.5
ndhC-trnV_p4	ndhC-trnV	4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.575098	50210	51388	1330-1350	1.000000	77.7
ndhC-trnV_p5	ndhC-trnV	5	CTCGGAGCTAAAAATCCCGA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	9.7	0.0	0.704436	50210	51388	1311-1331	1.000000	76.7
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	60369	61007	819-983	1.000000	80.9
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	60369	61007	751-915	1.000000	80.5
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	60369	61007	783-947	1.000000	80.4
ycf4-cemA_p4	ycf4-cemA	4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	60369	61007	765-929	1.000000	79.8
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	60369	61007	763-927	1.000000	79.5
psbB-psbT_p1	psbB-psbT	1	GGTGCTCGAACCTTGTTTAGA	CCCTAGAGTCGAGACTAAGAGG	21	22	47.6	54.5	58.2	58.3	0.0	5.7	3.501664	73503	73700	358-369	1.000000	54.0
psbB-psbT_p2	psbB-psbT	2	GGTGCTCGAACCTTGTTTAG	CCCTAGAGTCGAGACTAAGAGG	20	22	50.0	54.5	56.7	58.3	0.0	5.7	3.517332	73503	73700	358-369	1.000000	53.9
psbB-psbT_p3	psbB-psbT	3	GGTGCTCGAACCTTGTTTAGA	CCTAGAGTCGAGACTAAGAGGA	21	22	47.6	50.0	58.2	57.3	0.0	16.5	3.913231	73503	73700	357-368	1.000000	50.7
psbB-psbT_p4	psbB-psbT	4	GGTGCTCGAACCTTGTTTAGA	TCCCTAGAGTCGAGACTAAGAG	21	22	47.6	50.0	58.2	57.3	0.0	5.7	3.913231	73503	73700	359-370	1.000000	50.7
psbB-psbT_p5	psbB-psbT	5	GGTGCTCGAACCTTGTTTAG	CCTAGAGTCGAGACTAAGAGGA	20	22	50.0	50.0	56.7	57.3	0.0	16.5	3.928899	73503	73700	357-368	1.000000	50.6
trnR-trnN_p1	trnR-trnN	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	107297	107801	608-648	1.000000	82.9
trnR-trnN_p2	trnR-trnN	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	107297	107801	645-685	1.000000	82.3
trnR-trnN_p3	trnR-trnN	3	CAGAGGATTAGAGCACGTGG	GGTTGAGAACGGGAATGGAA	20	20	55.0	50.0	57.8	57.8	18.9	0.0	0.410108	107297	107801	647-687	1.000000	81.3
trnR-trnN_p4	trnR-trnN	4	GAACAGATCCAGTGGAGACG	GGTTGAGAACGGGAATGGAA	20	20	55.0	50.0	57.7	57.8	18.7	0.0	0.480028	107297	107801	684-724	1.000000	80.7
trnR-trnN_p5	trnR-trnN	5	CAGAGGATTAGAGCACGTGG	TCTCCCGTTGTTCCTCAGTA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.513605	107297	107801	612-652	1.000000	80.5
ycf1_p1	ycf1	1	TGCTGAGACTCAAAACAAAAAGA	GGATAGGGAAGAAGGATATCATGA	23	24	34.8	41.7	57.1	56.9	6.6	4.8	9.026468	109503	109622	183-195	1.000000	47.8
ycf1_p2	ycf1	2	TGCTGAGACTCAAAACAAAAAGAA	GGATAGGGAAGAAGGATATCATGA	24	24	33.3	41.7	57.7	56.9	6.6	4.8	9.397000	109503	109622	183-195	1.000000	47.8
ycf1_p3	ycf1	3	TGCTGAGACTCAAAACAAAAAGA	GGATAGGGAAGAAGGATATCATGAA	23	25	34.8	40.0	57.1	57.5	6.6	0.0	9.397696	109503	109622	183-195	1.000000	47.8
ycf1_p4	ycf1	4	TGCTGAGACTCAAAACAAAAAGA	GGATAGGGAAGAAGGATATCATG	23	23	34.8	43.5	57.1	55.5	6.6	4.8	9.421550	109503	109622	183-195	1.000000	47.8
ycf1_p5	ycf1	5	TGCTGAGACTCAAAACAAAAAG	GGATAGGGAAGAAGGATATCATGA	22	24	36.4	41.7	55.7	56.9	6.6	4.8	9.439093	109503	109622	183-195	1.000000	47.8
N4L36_pgt030-rpl32_p1	N4L36_pgt030-rpl32	1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	122848	123588	873-1041	1.000000	80.3
N4L36_pgt030-rpl32_p2	N4L36_pgt030-rpl32	2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	122848	123588	874-1042	1.000000	80.2
N4L36_pgt030-rpl32_p3	N4L36_pgt030-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	122848	123588	856-1024	1.000000	80.1
N4L36_pgt030-rpl32_p4	N4L36_pgt030-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	122848	123588	857-1025	1.000000	80.1
N4L36_pgt030-rpl32_p5	N4L36_pgt030-rpl32	5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.489250	122848	123588	883-1051	1.000000	79.3
rpl32-ndhF_p1	rpl32-ndhF	1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.6	58.0	0.0	17.5	0.397386	123754	124840	1262-1294	1.000000	79.0
rpl32-ndhF_p2	rpl32-ndhF	2	CCGCCCAATATCCTTTCCTT	TAGGAACTGGGAGTGGAAGG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.412351	123754	124840	1222-1254	1.000000	79.0
rpl32-ndhF_p3	rpl32-ndhF	3	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123754	124840	1260-1292	1.000000	78.9
rpl32-ndhF_p4	rpl32-ndhF	4	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	123754	124840	1261-1293	1.000000	78.9
rpl32-ndhF_p5	rpl32-ndhF	5	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.6	58.0	9.5	17.5	0.425347	123754	124840	1279-1311	1.000000	78.8
trnN-trnR_p1	trnN-trnR	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	127961	128465	608-648	1.000000	82.9
trnN-trnR_p2	trnN-trnR	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	127961	128465	645-685	1.000000	82.3
trnN-trnR_p3	trnN-trnR	3	GGTTGAGAACGGGAATGGAA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.8	57.8	0.0	18.9	0.410108	127961	128465	647-687	1.000000	81.3
trnN-trnR_p4	trnN-trnR	4	GGTTGAGAACGGGAATGGAA	GAACAGATCCAGTGGAGACG	20	20	50.0	55.0	57.8	57.7	0.0	18.7	0.480028	127961	128465	684-724	1.000000	80.7
trnN-trnR_p5	trnN-trnR	5	TCTCCCGTTGTTCCTCAGTA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.513605	127961	128465	612-652	1.000000	80.5