Hotspot

trnV(GAC)-rps12

IRa · 138330-139944 · 1615 bp

Back to Rehmannia

Pi 0.00124
MarkerSeek score 54.80
Primer available yes
Label trnV(GAC)-rps12

Feature Metadata

label_name
trnV(GAC)-rps12
feature_type
igs
region
IRa
length_bp
1615
variable_sites
5
pi
0.00124
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV(GAC)-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1711-1757 bp 1.0 77.3
trnV(GAC)-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1715-1761 bp 1.0 77.3
trnV(GAC)-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1710-1756 bp 1.0 77.3
trnV(GAC)-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1713-1759 bp 1.0 76.4
trnV(GAC)-rps12_p5 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1696-1742 bp 1.0 75.8