Hotspot

ndhD-psaC

SSC · 117236-117334 · 99 bp

Back to Rehmannia

Pi 0.0337

MarkerSeek score 62.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 99
variable_sites: 9
pi: 0.0337
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCAAGGAAAATGGTTCGTGG	CCCACGGATTTCTTGAGTGT	50.0	50.0	57.9	57.8	207-216 bp	1.0	84.8
ndhD-psaC_p2	GCAAGGAAAATGGTTCGTGG	AGGACTGCGTTGGTTGTAAG	50.0	50.0	57.9	58.1	245-254 bp	1.0	84.3
ndhD-psaC_p3	GCAAGGAAAATGGTTCGTGG	GGACTGCGTTGGTTGTAAGA	50.0	50.0	57.9	58.1	244-253 bp	1.0	84.3
ndhD-psaC_p4	GCAAGGAAAATGGTTCGTGG	GAGGACTGCGTTGGTTGTAA	50.0	50.0	57.9	58.1	246-255 bp	1.0	84.2
ndhD-psaC_p5	GCAAGGAAAATGGTTCGTGG	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.9	57.7	228-237 bp	1.0	83.1