Hotspot

rpl32-trnL(UAG)

SSC · 113618-114334 · 717 bp

Back to Rehmannia

Pi 0.00932

MarkerSeek score 51.30

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 717
variable_sites: 15
pi: 0.00932
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	GGGATATTGGGCAGCGTTAA	TACCATTTCACCATAGCGGC	50.0	50.0	58.0	58.0	845-858 bp	1.0	81.8
rpl32-trnL(UAG)_p2	GGGATATTGGGCAGCGTTAA	TAGGAGATGATATGCCGCCA	50.0	50.0	58.0	58.1	916-929 bp	1.0	81.5
rpl32-trnL(UAG)_p3	GGGCAGCGTTAAAAGCATTT	TACCATTTCACCATAGCGGC	45.0	50.0	57.9	58.0	837-850 bp	1.0	81.4
rpl32-trnL(UAG)_p4	GGGATATTGGGCAGCGTTAA	AGCGTGTCTACCATTTCACC	50.0	50.0	58.0	57.9	853-866 bp	1.0	81.4
rpl32-trnL(UAG)_p5	GGGCAGCGTTAAAAGCATTT	TAGGAGATGATATGCCGCCA	45.0	50.0	57.9	58.1	908-921 bp	1.0	81.1