Hotspot

rpl20-rps12

LSC · 69972-70776 · 805 bp

Back to Rehmannia

Pi 0.00331

MarkerSeek score 50.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 6
pi: 0.00331
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	CTCAACGTCGAGGAACATGT	50.0	50.0	58.0	57.9	870-902 bp	1.0	79.9
rpl20-rps12_p2	GTAAGTCTTGAATGGGCCCC	CTCAACGTCGAGGAACATGT	55.0	50.0	58.2	57.9	931-963 bp	1.0	78.3
rpl20-rps12_p3	TAAGTCTTGAATGGGCCCCT	CTCAACGTCGAGGAACATGT	50.0	50.0	58.3	57.9	930-962 bp	1.0	77.6
rpl20-rps12_p4	TTTGTTCTACGTCTCCGAGC	CTCAACGTCGAGGAACATGT	50.0	50.0	57.7	57.9	883-915 bp	1.0	77.4
rpl20-rps12_p5	TGTTCTACGTCTCCGAGCTA	CTCAACGTCGAGGAACATGT	50.0	50.0	57.6	57.9	881-913 bp	1.0	77.1