Hotspot

atpB-rbcL

LSC · 55480-56245 · 766 bp

Back to Rehmannia

Pi 0.00361

MarkerSeek score 50.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 7
pi: 0.00361
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCCCAGAACCGGAAGTAGTA	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	851 bp	1.0	77.1
atpB-rbcL_p2	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	927 bp	1.0	76.9
atpB-rbcL_p3	CGGACCGATAATTTGGACGA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	904 bp	1.0	76.5
atpB-rbcL_p4	TAATTTGGACGATACGCCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	896 bp	1.0	76.3
atpB-rbcL_p5	ACCGGACCGATAATTTGGAC	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	906 bp	1.0	75.6