Hotspot

psaA-ycf3

LSC · 42191-43142 · 952 bp

Back to Rehmannia

Pi 0.0377

MarkerSeek score 81.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 88
pi: 0.0377
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	948-1160 bp	1.0	89.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	929-1141 bp	1.0	89.1
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	877-1089 bp	1.0	88.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	858-1070 bp	1.0	88.6
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	881-1093 bp	1.0	88.4