Hotspot

rpoB-trnC(GCA)

LSC · 27183-28309 · 1127 bp

Back to Rehmannia

Pi 0.00977

MarkerSeek score 56.40

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 26
pi: 0.00977
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	ATCATTTGCATCCCCGAGC	ATGTTGATTAGGCGACACCC	52.6	50.0	58.3	58.0	1217-1277 bp	1.0	74.5
rpoB-trnC(GCA)_p2	ATCATTTGCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	52.6	50.0	58.3	58.1	1199-1259 bp	1.0	74.2
rpoB-trnC(GCA)_p3	CATTTGCATCCCCGAGCAT	ATGTTGATTAGGCGACACCC	52.6	50.0	58.6	58.0	1215-1275 bp	1.0	72.1
rpoB-trnC(GCA)_p4	ATCATTTGCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	52.6	50.0	58.3	58.4	1181-1241 bp	1.0	72.1
rpoB-trnC(GCA)_p5	CATTTGCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	58.6	58.1	1197-1257 bp	1.0	71.8