Hotspot

atpF-atpH

LSC · 13265-13635 · 371 bp

Back to Rehmannia

Pi 0.00385

MarkerSeek score 54.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 3
pi: 0.00385
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CCGAGGCAGAGGGAAAAATA	45.0	50.0	57.9	57.3	526-532 bp	1.0	76.4
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	527-533 bp	1.0	75.8
atpF-atpH_p3	AGTGGCCCAAAGAAACGAAA	CCGAGGCAGAGGGAAAAATA	45.0	50.0	58.2	57.3	528-534 bp	1.0	75.0
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.3	57.3	556-562 bp	1.0	74.7
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGA	45.0	47.6	57.9	57.7	521-527 bp	1.0	72.0