Hotspot

trnS(GCU)-trnG(UCC)

LSC · 8565-9311 · 747 bp

Back to Rehmannia

Pi 0.00656
MarkerSeek score 52.30
Primer available yes
Label trnS(GCU)-trnG(UCC)

Feature Metadata

label_name
trnS(GCU)-trnG(UCC)
feature_type
igs
region
LSC
length_bp
747
variable_sites
12
pi
0.00656
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(GCU)-trnG(UCC)_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 793-850 bp 1.0 56.6
trnS(GCU)-trnG(UCC)_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 792-849 bp 1.0 56.5
trnS(GCU)-trnG(UCC)_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 796-853 bp 1.0 55.5
trnS(GCU)-trnG(UCC)_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 795-852 bp 1.0 55.5
trnS(GCU)-trnG(UCC)_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 794-851 bp 1.0 55.5