Hotspot

ycf3

LSC · 141391-143333 · 1943 bp

Back to Rehderodendron

Pi 0.00144

MarkerSeek score 53.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1943
variable_sites: 7
pi: 0.00144
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATGGGGCCAACAAACAAT	GGATCATTTTCCCACGAGATG	45.0	47.6	58.3	56.7	2069-2108 bp	1.0	60.1
ycf3_p2	ATTTGACTGATGGGGCCAAC	GGATCATTTTCCCACGAGATG	50.0	47.6	58.4	56.7	2076-2115 bp	1.0	58.7
ycf3_p3	TTGGTGGTTTTTCCCAGAGG	GGATCATTTTCCCACGAGATG	50.0	47.6	57.9	56.7	2108 bp	0.8	53.5
ycf3_p4	TTGGTGGTTTTTCCCAGAGG	GGATCATTTTCCCACGAGATGT	50.0	45.5	57.9	58.5	2108 bp	0.8	51.9
ycf3_p5	TTGGTGGTTTTTCCCAGAGG	TCCCACGAGATGTAATGAGA	50.0	45.0	57.9	55.4	2099 bp	0.8	50.5