Hotspot

rps16

LSC · 102992-104102 · 1111 bp

Back to Rehderodendron

Pi 0.00396
MarkerSeek score 41.30
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1111
variable_sites
8
pi
0.00396
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GAATCATCACTCCCTCACTT AATTCCATACCGGCTCCAAA 45.0 45.0 54.4 57.2 1175-1184 bp 1.0 45.6
rps16_p2 GAATCATCACTCCCTCACTT TTCCATACCGGCTCCAAATT 45.0 45.0 54.4 57.2 1173-1182 bp 1.0 45.6
rps16_p3 TCATCACTCCCTCACTTTTTACA AATTCCATACCGGCTCCAAA 39.1 45.0 57.4 57.2 1172-1181 bp 1.0 45.5
rps16_p4 TCATCACTCCCTCACTTTTTACA TTCCATACCGGCTCCAAATT 39.1 45.0 57.4 57.2 1170-1179 bp 1.0 45.5
rps16_p5 GAATCATCACTCCCTCACTTT TTCCATACCGGCTCCAAATT 42.9 45.0 55.3 57.2 1173-1182 bp 1.0 44.4