Hotspot

rpoC1

LSC · 119052-121851 · 2800 bp

Back to Rehderodendron

Pi 0.0015

MarkerSeek score 57.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2800
variable_sites: 10
pi: 0.0015
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGAACCGGAGAGACCTGAAT	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.1	58.0	2961-2970 bp	1.0	79.5
rpoC1_p2	TGAACCGGAGAGACCTGAAT	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.1	57.9	2944-2953 bp	1.0	79.0
rpoC1_p3	TTGAACCGGAGAGACCTGAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.3	58.0	2962-2971 bp	1.0	77.6
rpoC1_p4	TTGAACCGGAGAGACCTGAA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.3	57.9	2945-2954 bp	1.0	77.2
rpoC1_p5	TGAACCGGAGAGACCTGAAT	TTCGATTGCTCGTTCGAGAA	50.0	45.0	58.1	57.7	2966-2975 bp	1.0	77.0