Hotspot

ndhD

SSC · 59924-61423 · 1500 bp

Back to Rehderodendron

Pi 0.00293

MarkerSeek score 47.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 11
pi: 0.00293
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAATTCCGCAATTGTGGCTT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	57.9	58.0	2112-2139 bp	1.0	79.8
ndhD_p2	CAATTCCGCAATTGTGGCTT	GGGACGGGTGTAAAGCTAAG	45.0	55.0	57.9	58.0	2141-2168 bp	1.0	79.7
ndhD_p3	CAATTCCGCAATTGTGGCTT	ATACCTTGGGACGGGTGTAA	45.0	50.0	57.9	58.0	2148-2175 bp	1.0	79.5
ndhD_p4	CAATTCCGCAATTGTGGCTT	GACGGGTGTAAAGCTAAGCA	45.0	50.0	57.9	57.9	2139-2166 bp	1.0	79.2
ndhD_p5	CAATTCCGCAATTGTGGCTT	TGCTTCTGCTCCAAGAACAG	45.0	50.0	57.9	58.1	2116-2143 bp	1.0	79.0