Hotspot

trnF-GAA-ndhJ

LSC · 147461-148154 · 694 bp

Back to Rehderodendron

Pi 0.00461

MarkerSeek score 50.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 8
pi: 0.00461
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	836-858 bp	1.0	80.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	800-822 bp	1.0	79.7
trnF-GAA-ndhJ_p3	ATGATGCATCGGGAATGGTC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	856-878 bp	1.0	79.3
trnF-GAA-ndhJ_p4	GATGATGCATCGGGAATGGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	857-879 bp	1.0	79.3
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	824-846 bp	1.0	79.0