Hotspot

ycf3-trnS-GGA

LSC · 143334-144176 · 843 bp

Back to Rehderodendron

Pi 0.0019

MarkerSeek score 52.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 4
pi: 0.0019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1006-1012 bp	1.0	66.9
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1007-1013 bp	1.0	66.9
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1027-1033 bp	1.0	66.4
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1028-1034 bp	1.0	66.4
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	977-983 bp	1.0	66.1