Hotspot

trnS-GCU-trnG-GCC

LSC · 106911-107576 · 666 bp

Back to Rehderodendron

Pi 0.0042

MarkerSeek score 61.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 7
pi: 0.0042
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	AGGAAACGGAAAGAGAGGGA	GCATTAAACCGAAAGACCCC	50.0	50.0	58.0	57.1	837-862 bp	1.0	73.0
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	GCATTAAACCGAAAGACCCC	45.0	50.0	58.0	57.1	783-808 bp	1.0	72.9
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	GCATTAAACCGAAAGACCCC	50.0	50.0	58.2	57.1	832-857 bp	1.0	72.0
trnS-GCU-trnG-GCC_p4	AGGAAACGGAAAGAGAGGGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	794-819 bp	1.0	70.6
trnS-GCU-trnG-GCC_p5	AGGAAACGGAAAGAGAGGGA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	794-819 bp	1.0	70.5