Hotspot

trnK-UUU-rps16

LSC · 102411-102991 · 581 bp

Back to Rehderodendron

Pi 0.00224

MarkerSeek score 61.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 3
pi: 0.00224
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCCTTGCTTCATCCA	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	707-995 bp	1.0	80.3
trnK-UUU-rps16_p2	ACGAATCCCTTGCTTCATCC	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	708-996 bp	1.0	80.2
trnK-UUU-rps16_p3	ATGGACGAATCCCTTGCTTC	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	712-1000 bp	1.0	80.2
trnK-UUU-rps16_p4	GGACGAATCCCTTGCTTCAT	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.0	58.1	710-998 bp	1.0	80.2
trnK-UUU-rps16_p5	CGAATCCCTTGCTTCATCCA	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.0	58.1	705-993 bp	1.0	80.2