Hotspot

rps15-ycf1

SSC · 67669-68020 · 352 bp

Back to Rehderodendron

Pi 0.00568

MarkerSeek score 37.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 352
variable_sites: 5
pi: 0.00568
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CTTCTTTTTCTTTTTGTGAAATAACTG	CCCGCGATTGAAAATTCGTT	25.9	45.0	55.2	57.7	423-453 bp	1.0	40.9
rps15-ycf1_p2	CTTTTTCTTTTTGTGAAATAACTG	CCCGCGATTGAAAATTCGTT	25.0	45.0	52.1	57.7	420-450 bp	1.0	40.9
rps15-ycf1_p3	CTTCTTTTTCTTTTTGTGAAATAACTG	ACCCGCGATTGAAAATTCGT	25.9	45.0	55.2	58.8	424-454 bp	1.0	40.9
rps15-ycf1_p4	CTTTTTCTTTTTGTGAAATAACTG	ACCCGCGATTGAAAATTCGT	25.0	45.0	52.1	58.8	421-451 bp	1.0	40.9
rps15-ycf1_p5	CTTCTTTTTCTTTTTGTGAAATAACTG	TACCCGCGATTGAAAATTCG	25.9	45.0	55.2	56.9	425-455 bp	1.0	40.9