Hotspot

rpl32-trnL-UAG

SSC · 57840-58558 · 719 bp

Back to Rehderodendron

Pi 0.00362

MarkerSeek score 53.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 719
variable_sites: 6
pi: 0.00362
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTCTTTCGAGCGGAACTTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	811-856 bp	1.0	79.1
rpl32-trnL-UAG_p2	CTCTTTCGAGCGGAACTTCA	TTAGAAGATAGCATGCCGCC	50.0	50.0	57.9	57.8	865-910 bp	1.0	78.7
rpl32-trnL-UAG_p3	CTCTTTCGAGCGGAACTTCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	804-849 bp	1.0	78.6
rpl32-trnL-UAG_p4	CTCTTTCGAGCGGAACTTCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	810-855 bp	1.0	78.5
rpl32-trnL-UAG_p5	CTCTTTCGAGCGGAACTTCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	809-854 bp	1.0	78.5