Hotspot

ndhF-rpl32

SSC · 56897-57677 · 781 bp

Back to Rehderodendron

Pi 0.00333

MarkerSeek score 59.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 781
variable_sites: 6
pi: 0.00333
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGAAGAAGCCCCACTCC	AAAACGCTTTTAAGGCTGCC	50.0	45.0	57.7	57.9	961-1137 bp	1.0	77.2
ndhF-rpl32_p2	AAGAAGAAGCCCCACTCCTA	AAAACGCTTTTAAGGCTGCC	50.0	45.0	57.7	57.9	959-1135 bp	1.0	76.8
ndhF-rpl32_p3	AAAAGAAGAAGCCCCACTCC	AGGCTGCCCAATATCCTTTC	50.0	50.0	57.7	57.6	949-1125 bp	1.0	75.4
ndhF-rpl32_p4	AAAAGAAGAAGCCCCACTCC	GCTGCCCAATATCCTTTCCT	50.0	50.0	57.7	57.6	947-1123 bp	1.0	75.4
ndhF-rpl32_p5	AAAGAAGAAGCCCCACTCCT	AAAACGCTTTTAAGGCTGCC	50.0	45.0	58.5	57.9	960-1136 bp	1.0	75.1