Hotspot

psbE-petL

LSC · 9534-10814 · 1281 bp

Back to Rehderodendron

Pi 0.00219

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 7
pi: 0.00219
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.9	57.3	1442-1447 bp	1.0	58.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	1365-1370 bp	1.0	57.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	TGAGGTTATAGTTAAAGCCGCT	45.0	40.9	58.2	57.3	1451-1456 bp	1.0	57.0
psbE-petL_p4	TGCTGACGAATAACCAACCC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.2	57.3	1456-1461 bp	1.0	56.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	1450-1455 bp	1.0	56.9