Hotspot

ycf4-cemA

LSC · 5424-5953 · 530 bp

Back to Rehderodendron

Pi 0.00321

MarkerSeek score 59.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 4
pi: 0.00321
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	510-1053 bp	1.0	83.1
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	524-1067 bp	1.0	82.4
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	522-1065 bp	1.0	82.1
ycf4-cemA_p4	GCCATTCCCTTGACTCGTAC	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.3	58.1	576-1119 bp	1.0	81.0
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	AGAAATCCACCAGGGCAAAA	45.0	45.0	58.1	57.6	509-1052 bp	1.0	80.5