Hotspot

ycf1

SSC · 130735-136434 · 5700 bp

Back to Reevesia

Pi 0.00172

MarkerSeek score 34.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5700
variable_sites: 26
pi: 0.00172
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGGTGCTATAGCTATAAGGGG	TCAAGAAAGCAACCCATCCA	52.4	45.0	57.2	57.3	256 bp	1.0	62.7
ycf1_p2	CGGTGCTATAGCTATAAGGGG	TCAAGAAAGCAACCCATCCAA	52.4	42.9	57.2	58.0	256 bp	1.0	59.7
ycf1_p3	GGTGCTATAGCTATAAGGGGG	TCAAGAAAGCAACCCATCCA	52.4	45.0	56.8	57.3	255 bp	1.0	59.6
ycf1_p4	TGCTATAGCTATAAGGGGGTCC	TCAAGAAAGCAACCCATCCA	50.0	45.0	58.3	57.3	253 bp	1.0	58.9
ycf1_p5	CGGTGCTATAGCTATAAGGGG	AGAAAGCAACCCATCCAATCA	52.4	42.9	57.2	57.8	253 bp	1.0	58.7