Hotspot

rps19

LSC · 90393-90671 · 279 bp

Back to Reevesia

Pi 0.00179

MarkerSeek score 23.20

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 1
pi: 0.00179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	CTGCGGCTTGACTAATGACT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	777-803 bp	1.0	83.0
rps19_p2	CTGCGGCTTGACTAATGACT	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	975-1001 bp	1.0	82.3
rps19_p3	CTGCGGCTTGACTAATGACT	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.0	58.1	1057-1083 bp	1.0	81.9
rps19_p4	CTGCGGCTTGACTAATGACT	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	1048-1074 bp	1.0	81.9
rps19_p5	TAACTCCGTCCTCTAGCTCG	AAAAGTTTGGGTAGAGCCGG	55.0	50.0	58.0	58.1	1030-1056 bp	1.0	81.8