Hotspot

ndhF-rpl32

SSC · 118370-119650 · 1281 bp

Back to Reevesia

Pi 0.0039

MarkerSeek score 56.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1281
variable_sites: 10
pi: 0.0039
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	GCTTTCAACGAAGCCCAATA	45.0	45.0	58.0	56.7	1464-1474 bp	1.0	70.6
ndhF-rpl32_p2	TTGGAAGTGGCACAAAAGGT	GCTTTCAACGAAGCCCAATA	45.0	45.0	58.1	56.7	1417-1427 bp	1.0	69.9
ndhF-rpl32_p3	TGTTGCCGTCGGAAAAAGTA	TCAACGAAGCCCAATATCCT	45.0	45.0	58.0	56.6	1460-1470 bp	1.0	69.8
ndhF-rpl32_p4	TTTGTTGCCGTCGGAAAAAG	GCTTTCAACGAAGCCCAATA	45.0	45.0	57.8	56.7	1466-1476 bp	1.0	69.4
ndhF-rpl32_p5	TTGGAAGTGGCACAAAAGGT	TCAACGAAGCCCAATATCCT	45.0	45.0	58.1	56.6	1413-1423 bp	1.0	69.1