Hotspot

rps19-rpl2

IRb · 90672-90830 · 159 bp

Back to Reevesia

Pi 0.0228

MarkerSeek score 52.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 159
variable_sites: 7
pi: 0.0228
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AATGATTTGCTACAAAAGGATT	TTGATTCTTCGTCGCCGTAG	27.3	50.0	52.6	58.1	204-230 bp	1.0	46.6
rps19-rpl2_p2	ATGATTTGCTACAAAAGGATTT	TTGATTCTTCGTCGCCGTAG	27.3	50.0	52.6	58.1	203-229 bp	1.0	46.6
rps19-rpl2_p3	AAATGATTTGCTACAAAAGGAT	TTGATTCTTCGTCGCCGTAG	27.3	50.0	52.6	58.1	205-231 bp	1.0	46.5
rps19-rpl2_p4	AATGATTTGCTACAAAAGGATT	TTGGGGTTATCCTGCACTTG	27.3	50.0	52.6	57.8	262-288 bp	1.0	46.0
rps19-rpl2_p5	ATGATTTGCTACAAAAGGATTT	TTGGGGTTATCCTGCACTTG	27.3	50.0	52.6	57.8	261-287 bp	1.0	46.0