Hotspot

rpl33-rps18

LSC · 73610-74019 · 410 bp

Back to Reevesia

Pi 0.00509

MarkerSeek score 56.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 8
pi: 0.00509
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	560-593 bp	1.0	78.8
rpl33-rps18_p2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	559-592 bp	1.0	76.4
rpl33-rps18_p3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	561-594 bp	1.0	76.4
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.5	58.6	557-590 bp	1.0	75.7
rpl33-rps18_p5	ACGCCTAGTCGATTGGAATT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	551-584 bp	1.0	75.2