Hotspot

trnT-UGU-trnL-UAA

LSC · 49973-51491 · 1519 bp

Back to Reevesia

Pi 0.00283

MarkerSeek score 52.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1519
variable_sites: 12
pi: 0.00283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CAAATGCGAGGCTCTAACCT	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1550-1577 bp	1.0	84.3
trnT-UGU-trnL-UAA_p2	CAAATGCGAGGCTCTAACCT	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	1558-1585 bp	1.0	83.8
trnT-UGU-trnL-UAA_p3	GCATTACAAATGCGAGGCTC	ACCAATTTCGCCATATCCCC	50.0	50.0	57.9	58.0	1556-1583 bp	1.0	83.7
trnT-UGU-trnL-UAA_p4	CAAATGCGAGGCTCTAACCT	CCAAGGCTCAATCCAATCCA	50.0	50.0	58.0	57.9	1588-1615 bp	1.0	83.2
trnT-UGU-trnL-UAA_p5	CAAATGCGAGGCTCTAACCT	ACCAAGGCTCAATCCAATCC	50.0	50.0	58.0	57.9	1589-1616 bp	1.0	83.2