Hotspot

rpoB-trnC-GCA

LSC · 28034-29287 · 1254 bp

Back to Reevesia

Pi 0.00239

MarkerSeek score 49.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 7
pi: 0.00239
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCTGTTAAGCCCCGATCC	CCGGATTTGAACTGGGGAAT	55.0	50.0	58.0	57.9	1399-1417 bp	1.0	79.9
rpoB-trnC-GCA_p2	TTTCCCCATTTTCATCCCCG	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.1	57.9	1324-1342 bp	1.0	79.2
rpoB-trnC-GCA_p3	CCGATCCATGAACCCACAAA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.2	57.9	1386-1404 bp	1.0	78.9
rpoB-trnC-GCA_p4	CCATTTTCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.3	57.9	1319-1337 bp	1.0	77.9
rpoB-trnC-GCA_p5	TTAAGCCCCGATCCATGAAC	CCGGATTTGAACTGGGGAAT	50.0	50.0	57.7	57.9	1393-1411 bp	1.0	77.5