Hotspot

ycf1

SSC · 125247-130631 · 5385 bp

Back to Reaumuria

Pi 0.021

MarkerSeek score 52.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5385
variable_sites: 113
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAATCTTCAGTCCATCCGA	ATCCACCGGAAGAAGTAGCT	50.0	50.0	57.1	58.1	959 bp	1.0	74.4
ycf1_p2	CGAATCTTCAGTCCATCCGA	ATGCGTGCTCAAAGACGTAA	50.0	45.0	57.1	58.2	885 bp	1.0	73.9
ycf1_p3	CGAATCTTCAGTCCATCCGA	GATCCACCGGAAGAAGTAGC	50.0	55.0	57.1	57.8	960 bp	1.0	73.6
ycf1_p4	TCGAATCTTCAGTCCATCCG	ATCCACCGGAAGAAGTAGCT	50.0	50.0	57.1	58.1	960 bp	0.5	52.0
ycf1_p5	TCGAATCTTCAGTCCATCCG	ATGCGTGCTCAAAGACGTAA	50.0	45.0	57.1	58.2	886 bp	0.5	51.4