Hotspot

ndhF

SSC · 111947-114172 · 2226 bp

Back to Reaumuria

Pi 0.0175

MarkerSeek score 54.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 39
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGAGTGAATGGAAAGGACA	TTTGACACATTGGAGTCCCG	50.0	50.0	57.5	58.1	3000 bp	0.5	55.3
ndhF_p2	CCGAGTGAATGGAAAGGACAA	TTTGACACATTGGAGTCCCG	47.6	50.0	58.2	58.1	3000 bp	0.5	49.4
ndhF_p3	CCGAGTGAATGGAAAGGACA	TTGACACATTGGAGTCCCG	50.0	52.6	57.5	57.4	2999 bp	0.5	43.2
ndhF_p4	TCTGGTTTGAAAAACCCCTTCT	TTTGACACATTGGAGTCCCG	40.9	50.0	58.0	58.1	2848 bp	0.5	43.0
ndhF_p5	TCTTCTGGTTTGAAAAACCCCT	TTTGACACATTGGAGTCCCG	40.9	50.0	58.0	58.1	2851 bp	0.5	43.0